[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary and its regulation function.[Method] cDNA library of miRNA in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary were established.After Solexa high-throughput sequencing of miRNA in the cDNA library,miRNA in anterior pituitary of bulls was identified.miRNA-181b with differential expression were selected from the sequencing results.By real-time quantitative RT-PCR,the expression laws of miRNA-181b in the anterior pituitary of Yanbian Cattle in different growth period was validated.And the target genes of miRNA-181b were forecast by using TargetScanS prediction software.[Result] The expression quantity of miRNA-181b had great difference in cattle＇s anterior pituitary different growth periods.The expression quantity of miRNA-181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle.The binding of miRNA-181b with 838-844 bases in 3＇ untranslated region of FSHβ gene was specific and the binding base sites were UGAAUGUA.[Conclusion] This research provided the theoretical basis for the transcription regulation research of FSHβ.
