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王国华

作品数:10 被引量:38H指数:4
供职机构:西北农林科技大学动物科技学院更多>>
发文基金:国家高技术研究发展计划博士科研启动基金更多>>
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10 条 记 录,以下是 1-7
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透明质酸对山羊卵母细胞体外成熟和孤雌胚胎发育的影响被引量:2
2007年
在无血清培养体系中添加不同浓度的HA,探讨HA浓度对山羊卵母细胞体外成熟和早期胚胎发育的影响。试验结果表明,当HA浓度在1.0和1.5 mg/ml时,卵母细胞成熟率(分别为74.5%和76.3%)显著高于对照组(P〈0.05),且高于其他各组,说明HA可以促进卵母细胞成熟,且浓度在1.0~1.5 mg/ml时最有利于卵母细胞成熟;当HA浓度为1.5 mg/ml时,卵裂率(85.7%)和囊胚率(44.4%)均显著高于对照组,与其他各组比较,均达最优值,说明HA可以促进早期胚胎发育,且浓度为1.5 mg/ml时,最有利于早期胚胎发育。
蒋永海孙亚东张林王国华张涌
关键词:透明质酸山羊卵母细胞体外成熟胚胎发育
多种外源激素联合诱导德国牧羊犬同期发情的效果研究
2022年
为探讨外源激素对德国牧羊犬同期发情和相关指标的影响,该试验选取36只德国牧羊犬母犬(年龄3~4岁),随机分为A、B、C、D 4个组,每组3个重复,每个重复3只。A、B、C组分别采用激素PG/PMSG/HCG、PG/PG600/HCG、PG/FG600/HCG进行处理,D组不使用任何激素处理;分别测定其同期发情率、受胎率、产仔数,并对阴道上皮细胞进行涂片监测、对卵泡的发育进行超声监测。结果表明:B组同期发情率最高,为90%,与其它组相比差异显著(P<0.05);A、B、C组卵泡数量较对照组多,平均排卵时间比对照组缩短;使用激素和未使用激素组犬的卵泡平均直径无显著差异;C组受胎率最高,为100%,与其它组相比差异显著(P<0.05);A组产仔数最高,平均为8只,与其他组相比差异显著(P<0.05)。可见,外源激素PG/PMSG/HCG组合诱导德国牧羊犬产仔数最高,可以为德国牧羊犬繁育提供一定临床指导。
修福晓王国华王国华
关键词:外源激素德国牧羊犬同期发情受胎率产仔数
山羊卵母细胞和孤雌激活胚胎体外无血清培养的研究
本文为研究山羊卵母细胞和孤雌激活胚胎在无血清成熟液和培养液中的成熟、发育情况而做了两个实验:(1)山羊卵母细胞无血清体外成熟培养;(2)山羊孤雌激活胚胎无血清体外培养。实验的目的是为避免血清对卵母细胞和胚胎的副作用和不利...
王国华
关键词:山羊卵母细胞孤雌激活囊胚发育率体外成熟培养囊胚率成熟率
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“胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响被引量:4
2008年
[目的]为提高山羊卵母细胞及胚胎体外培养的效率,探索无血清体外培养体系。[方法]在目前卵母细胞体外成熟及胚胎培养所用的常规培养液中添加1%ITS(胰岛素-转铁蛋白-硒钠)或以1%ITS分别替代2种培养液中FBS,对山羊卵母细胞及孤雌激活胚胎进行体外培养,检测ITS对其发育率的影响。[结果]添加ITS到卵母细胞成熟液中,对卵母细胞成熟率没有明显提高,但显著提高了其激活后孤雌胚胎的囊胚率(58.06%vs.48.19%);以ITS替代成熟液中FBS,取得了与FBS组相似的成熟率、卵裂率和囊胚率。添加ITS到胚胎培养液中,显著提高了山羊孤雌胚胎的囊胚率(68.30%vs.56.82%);以ITS替代胚胎培养液中FBS,卵裂率与FBS组无显著差异,囊胚率显著低于FBS组(42.33%vs.56.82%)。[结论]ITS对山羊卵母细胞体外成熟及胚胎早期发育均有促进作用;另外,ITS可以替代卵母细胞成熟培养液中的血清,作为无血清培养体系用于相关研究。
刘凤军张玉玲杨自军王国华张涌
关键词:卵母细胞孤雌激活山羊
Effect of Insulin-Transferrin-Selenium on Goat Oocytes Maturation and Embryo Development被引量:9
2008年
[Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.[Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.[Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn’t change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).[Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.
刘凤军张玉玲杨自军王国华张涌
关键词:OOCYTEPARTHENOGENESIS
体细胞核移植法获得转人乳铁蛋白基因克隆山羊妊娠的研究被引量:8
2008年
[目的]探讨供体细胞类型、移植胚胎发育阶段、数量及部位对山羊转基因克隆效率的影响。[方法]利用体细胞核移植技术将转染人乳铁蛋白基因hLF的山羊胎儿成纤维细胞(GFF)和乳腺上皮细胞(GMGE)移植到MII期去核卵母细胞内,经电融合、激活、体外培养后,2-8细胞期克隆胚被移植到同期发情山羊的输卵管内,囊胚被移植到子宫角内。[结果]GFF与GMGE的妊娠率相近(输卵管移植妊娠率分别为26.47%及20.00%);在GFF,输卵管移植的妊娠率与子宫角内移植妊娠率接近(分别为26.47%及25.00%),输卵管移植胚胎平均数为21.2组的妊娠率显著高于5.93组和9.64组(40.00%及26.67%,21.43%)。[结论]供体细胞类型、移植胚胎的发育阶段及移植部位对山羊转基因克隆效率的影响不大,但对于输卵管移植,受体羊移植胚胎数量对妊娠率有明显的影响。此外,该研究还提示了利用成年羊乳腺上皮细胞制作转基因动物的可行性。
刘凤军张玉玲杨自军陈兴启孙达权王国华张涌
关键词:体细胞核移植转基因人乳铁蛋白基因乳腺上皮细胞
Pregnancies Resulting from Transgenic Embryos with Human Lactoferrin Gene Produced by Somatic Cell Nuclear Transfer被引量:3
2008年
[Objective] The aim of this study is to understand the effects of donor cell type,embryo stage,number and transfer position on the efficiency of goat transgenic clone.[Method] Using somatic cell nuclear transfer technology,the single goat fetal fibroblasts(GFF)and mammary gland epithelial cells(GMGE)harboring human lactoferrin(hLF)gene were transferred to the enucleated oocyte.Reconstructed karyoplast-cytoplast couplets were fused,activated,and cultured in vitro.Embryos at 2-8 cell stage were transferred into oviduct of synchronized recipients,and blastocysts were transferred into uterine horn.[Result] The pregnancy rate was similar between GFF and GMGE(oviduct transfer:26.47% vs.20.00%),and between oviduct transfer and uterine horn transfer(26.47% vs.25.00%)for GFF group;pregnancy rate in the group with the mean number of embryo transferred per recipient of 21.2 was significantly higher than in those the 5.93 group and 9.64 group(40.00% vs.26.67% and 21.43%).[Conclusion] These results indicate that pregnancy rate of goat transgenic clone couldn't be affected by donor cell type,embryo stage and transfer position but be done by the number of embryo transferred per recipient.In addition,the study also suggests the feasibility of making transgenic goat using GMGE as donor cells.
刘凤军张玉玲杨自军陈兴启孙达权王国华张涌
关键词:SOMATICTRANSGENELACTOFERRINMAMMARYGLAND
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