For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 column （150 mm×4.6 mm, 5 μm）; column temperature： 35 ℃; mobile phase： methanol water （65：35, v/v）; flow rate： 1.0 mL/min; detection wavelength： 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of （0.0998 0.8982） mg/mL （r = 0.9999） and （0.1474-1.3266） mg/mL （r = 0.9999）, respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.
A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS-4 column（4.6 mm×150 mm, 5 μm） with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity（r20.9991）, precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of Polygonum aviculare L.
