Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.
The combinations of Hyphantria cunea NPV(HcNPV) and three different fluorescent brighteners BA,VBL,CBS-X were added to the artificial diet and the influence of synergists in concentration 25 ℃ and 14L/10D(h).The results showed that the fluorescent brighteners BA and VBL had higher insecticidal activity,CBS-X had not significant synergistic effect.
