您的位置: 专家智库 > >

王文亚

作品数:4 被引量:27H指数:3
供职机构:云南农业大学农学与生物技术学院更多>>
发文基金:国家自然科学基金更多>>
相关领域:生物学农业科学更多>>

文献类型

  • 4篇中文期刊文章

领域

  • 2篇生物学
  • 2篇农业科学

主题

  • 1篇植物
  • 1篇生物合成
  • 1篇花色
  • 1篇花色苷
  • 1篇基因
  • 1篇基因组
  • 1篇基因组DNA
  • 1篇高等植物
  • 1篇PLANT
  • 1篇PLANTS
  • 1篇STRENG...
  • 1篇THREE
  • 1篇CULTIV...
  • 1篇DROUGH...
  • 1篇IN_VIT...
  • 1篇ISOLAT...
  • 1篇MEC
  • 1篇SPOT
  • 1篇ROUND
  • 1篇PHYSIO...

机构

  • 4篇云南农业大学
  • 2篇文山市苗乡三...
  • 1篇文山学院
  • 1篇文山县苗乡三...

作者

  • 4篇赵昶灵
  • 4篇王文亚
  • 2篇王崇德
  • 2篇文国松
  • 2篇翁晨
  • 1篇陈中坚
  • 1篇李孙文
  • 1篇陈文龙
  • 1篇支伟特
  • 1篇孙艳
  • 1篇唐小华

传媒

  • 2篇Agricu...
  • 1篇江苏农业科学
  • 1篇中国农学通报

年份

  • 1篇2015
  • 2篇2014
  • 1篇2013
4 条 记 录,以下是 1-4
排序方式:
高等植物花色苷生物合成调控的研究进展被引量:12
2013年
高等植物花色苷的合成是一个复杂的过程,受多种因素的影响。系统地从物理因子、化学因子、生物因子等外部因素,及生长发育阶段、植物生长物质等内部因素两方面综述了调控高等植物花色苷生物合成的因素及其机理的研究进展,并分析了各种外、内部作用因子的综合性包括作用的协调性与机理的一致性。最后,提出了高等植物花色苷生物合成调控研究中存在的一些问题,并探讨了利用各种因子人工调控花色苷合成以提高果实营养价值、花卉观赏价值及植物抗逆性。
陈文龙赵昶灵支伟特陈中坚缪康汝王文亚翁晨
关键词:高等植物花色苷生物合成
5种提取三七基因组DNA方法的比较被引量:9
2014年
用高盐低pH值法、尿素法、CTAB(十六烷基三甲基溴化铵)法、SDS(十二烷基磺酸钠)法、PVP(聚乙烯吡咯烷酮)法提取一年生三七叶的基因组DNA,用紫外分光光度法测定提取的DNA浓度,用琼脂糖凝胶电泳法测定提取的DNA质量。结果表明:用CTAB法提取的基因组DNA含量、得率最高,分别为41.434 3~43.233 6μg/mL、114.829 5~127.282 4μg/g,且D260 nm/D280 nm均在标准值范围内,D260 nm/D230 nm小于1.9,有RNA污染;SDS法、PVP法、尿素法也可以提取基因组DNA,但含量较低;高盐低pH值法提取的DNA浓度最低,电泳图基本看不到明显条带。将这5种方法进行对比,最终确定CTAB法为三七基因组DNA的最佳提取方法。
白雪嵩赵昶灵陈中坚翁晨王文亚
关键词:基因组DNA
Physiological Mechanism for Anthocyanins to Strengthen the Drought Tolerance of Plants被引量:4
2014年
This paper summarized the possible physiological mechanism by which anthocyanins strengthen the tolerance of plants to drought. Drought stress can in-duce plant cel s to synthesize and accumulate anthocyanins. The photochemical properties, subcel ular accumulation sites and spatial distributions in plant organs and tissues of anthocyanins determine their function of strengthening plant tolerance, which is realized by three possible physiological mechanisms: (1) anthocyanins and their chelated metal ions can optimize the osmoregulation ability of the plant cel s by directly acting as the osmoregulation substances of the cel s, (2) anthocyanins with suitable spatial locations can reduce the photoinhibition of the plants under drought stresses, (3) anthocyanins can effectively maintain and improve the active oxygen-scavenging capacity of the plant cel s under drought conditions. Therein, that the anthocyanins enhance the antioxidant capacity of the plant cel s under drought stresses is probably the main reason for the anthocyanins to strengthen the drought tolerance of plants. This review could provide a reference for the mechanism re-search of the drought resistance and the breeding of the drought-resistant cultivars for the plants holding the ability to synthesize and accumulate anthocyanins.
唐小华赵昶灵文国松王文亚王崇德孙艳白雪嵩
Studies on the Isolation, Identification and In Vitro Growth Rates of the Three Pathogenic Fungi from Panax notoginseng Cultivated in Wenshan Eparchy被引量:2
2015年
Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar (PDA) medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space (ITS) se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread faste
王文亚赵昶灵陈中坚文国松魏富刚龙廷菊李孙文王崇德
共1页<1>
聚类工具0