Hypsizygus marmoreus has a significant dependence on environment at the mycelium growth stage. ln order to col ect, process and display real-time data of temperature, humidity, CO2 concentration in cultivation room, along-distance environ-ment monitoring system with multi-technology is designed. The curves of tempera-ture, humidity and CO2 concentration, fitted by the MATLAB data fitting toolbox with the experimental data, reflected the changing trend of environmental factors. The mathematical model of CO2 concentration was established by regression analysis, and the regulation strategy that the ventilation period of half an hour and the venti-lation duration of 5 minutes was put forward.