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李小凤

作品数:2 被引量:5H指数:2
供职机构:河北农业大学植物保护学院更多>>
发文基金:河北省自然科学基金更多>>
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不同菌源诱导免疫的黄粉虫抗菌肽提取物对26种病原菌的抑菌活性被引量:2
2011年
[目的]探索经不同菌源诱导黄粉虫产生的免疫物质——抗菌肽对病原菌是否存在活性差异。[方法]通过饲细菌、针刺饲真菌法诱导饥饿处理过的黄粉虫幼虫产生免疫反应,研磨、冷冻离心法获得抗菌肽提取物,采用抑菌圈法测定其对26种病原菌抑菌活性,利用三等分法划分4种类型评价抑菌活性及抑菌谱。[结果]经不同菌源诱导黄粉虫所产生的抗菌肽抑菌活性及抑菌谱上均存在明显差异。[结论]生防菌源在诱导昆虫体液免疫物质——抗菌肽表达上具有明显优势。
谢咸升董建臻李静李永利王圆圆李小凤张亚南
关键词:黄粉虫诱导免疫抗菌肽抑菌活性抑菌谱
Analysis of the Antimicrobial Activity of Antimicrobial Peptides Extracted from Immunized Tenebrio molitor Induced by Different Microbial Treatments Against 26 Pathogens被引量:3
2010年
[Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.[Method]By inducing T.molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking,the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.[Result]Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers.[Conclusion]Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.
谢咸升董建臻李静李永利王圆圆李小凤张亚南
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