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康大海

作品数:2 被引量:21H指数:2
供职机构:中国水产科学研究院黑龙江水产研究所农业部北方鱼类生物工程育种重点开放实验室更多>>
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Microsatellite DNA Marker Analysis of Genetic Diversity in Wild Common Carp (Cyprinus carpio L.) Populations被引量:12
2007年
Thirty microsatellite loci were used for analyzing six wild populations of common carp (Cyprinus carpio L.). Observed (Ho) and expected (He) heterozygosity values, polymorphic information content (PIC), and number of effective alleles (Ae) were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package (version 3.63). There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1) the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2) the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.
李大宇康大海殷倩茜孙效文梁利群
关键词:MICROSATELLITE
红鳍东方鲀F1代混养群体的基因型鉴定及QTL分析被引量:9
2008年
利用微卫星标记的方法,分别对3个家系红鳍东方鲀的基因型进行鉴定,并通过此方法对3个家系混养后的混合群体进行区分,利用区分的结果与混合群体的生长性状指标进行QTL分析,结果为F0041中的基因型153/150,标记F0254中的基因型325/335,标记F0220中的基因型175/189,标记F0254中的基因型325/335与河鲀的体长、体质量表型值正相关,标记F0041基因型153/143,F0151标记中的250/250基因型,标记F0157中的基因型167/198,标记F0220中的基因型175/205与河鲀的体长、体质量表型值负相关。以期采用此方法能够为今后的亲子鉴定、个体识别及家系识别提供理论基础。
刘秀云康大海孙效文孟雪松
关键词:红鳍东方鲀基因型QTL
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