BACKGROUND:Heatstroke has become a common emergency event in hospitals.Procalcitonin(PCT)is used as a biomarker of infection in the emergency department(ED),but its role in rhabdomyolysis(RM)following exertional heatstroke(EHS)remains unclear.METHODS:A retrospective cohort study enrolled patients with EHS from the intensive care unit(ICU).We collected RM biomarkers,inflammation markers,critical disease scores at admission,24 h,48 h,and discharge,and 90-day mortality.Correlation analysis,linear regression and curve fi tting were used to identify the relationship between PCT and RM.RESULTS:A total of 162 patients were recruited and divided into RM(n=56)and non-RM(n=106)groups.PCT was positively correlated with myoglobin(Mb),acute hepatic injury,disseminated intravascular coagulation(DIC),Sequential Organ Failure Assessment(SOFA)score,and Acute Physiology and Chronic Health Evaluation II(APACHE II)score,with correlation coefficients of 0.214,0.237,0.285,0.454,and 0.368,respectively(all P<0.05).Interestingly,the results of curve fi tting revealed a nonlinear relationship between PCT and RM,and a two-piecewise linear regression model showed that PCT was related to RM with an odds ratio of 1.3 and a cut-off of<4.6 ng/mL.Survival analysis revealed that RM was associated with higher mortality compared to non-RM cases(P=0.0093).CONCLUSION:High serum PCT concentrations are associated with RM after EHS in critically ill patients.Elevated PCT concentrations should be interpreted cautiously in patients with EHS in the ED.
Li ZhongFeifei ShuaiConglin WangLipeng HanZhifeng LiuMing Wu
BACKGROUND Necrotising enterocolitis(NEC)is a critical gastrointestinal emergency affecting premature and low-birth-weight neonates.Serum amyloid A(SAA),procalcitonin(PCT),and high-mobility group box 1(HMGB1)have emerged as potential biomarkers for NEC due to their roles in inflammatory response,tissue damage,and immune regulation.AIM To evaluate the diagnostic value of SAA,PCT,and HMGB1 in the context of NEC in newborns.METHODS The study retrospectively analysed the clinical data of 48 newborns diagnosed with NEC and 50 healthy newborns admitted to the hospital.Clinical,radiological,and laboratory findings,including serum SAA,PCT,and HMGB1 Levels,were collected,and specific detection methods were used.The diagnostic value of the biomarkers was evaluated through statistical analysis,which was performed using chi-square test,t-test,correlation analysis,and receiver operating characteristic(ROC)analysis.RESULTS The study demonstrated significantly elevated levels of serum SAA,PCT,and HMGB1 Levels in newborns diagnosed with NEC compared with healthy controls.The correlation analysis indicated strong positive correlations among serum SAA,PCT,and HMGB1 Levels and the presence of NEC.ROC analysis revealed promising sensitivity and specificity for serum SAA,PCT,and HMGB1 Levels as potential diagnostic markers.The combined model of the three biomarkers demonstrating an extremely high area under the curve(0.908).CONCLUSION The diagnostic value of serum SAA,PCT,and HMGB1 Levels in NEC was highlighted.These biomarkers potentially improve the early detection,risk stratification,and clinical management of critical conditions.The findings suggest that these biomarkers may aid in timely intervention and the enhancement of outcomes for neonates affected by NEC.
Procalcitonin(PCT)is a promising biomarker for identification of the origin and severity of sepsis,which is a deadly body infection.However,traditional diagnostic tools exhibited challenges in complicated instruments,sensitivity and time consuming.Herein,we created a highly sensitive and selective surface-enhanced Raman scattering(SERS)platform for PCT monitoring based on flower-like Bi2WO6-graphene(Bi2WO6-GO),which was created as a chemical mechanism(CM)-based SERS substrate with high stability as well as a remarkable enhancement factor(EF)value of 2.07×10^(8).The high EF value was primarily attributed to the efficient charge transfer(CT)between Bi_(2)WO_(6)-GO and 4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en)vinyl)phenyl)boronic acid(BP)as a Raman reporter.The BP molecule was designed to play double key roles as a Raman reporter as well as a recognition probe.Owing to the specially designed BP molecule recognition of PCT and the high SERS effects of BP on Bi_(2)WO_(6)-GO,the developed SERS platform was employed for ultrasensitive and selective PCT quantification with a limit of detection down to 0.31 pg/mL in less than 8 min.The developed platform was also successfully utilized for early monitoring in sepsis rats,demonstrating practical potential for pathogene screening.