搜索到4326篇“ METHIONINE“的相关文章
蛋氨酸对反刍动物肝脏健康的影响及其机制
2024年
蛋氨酸是反刍动物的第一限制性氨基酸,具有调节糖和脂代谢、抗炎、抗氧化等多重生理作用。因瘤胃微生物的降解作用,生产中常采用过瘤胃蛋氨酸(RPM)的形式为反刍动物补充蛋氨酸。作为动物机体营养物质的代谢中枢和重要的免疫器官,肝脏健康与动物生产性能和健康密切相关。研究发现,蛋氨酸可通过对肝脏代谢及其功能进行调控,进而可维护反刍动物的肝脏健康。因此,本文对蛋氨酸的理化性质及营养作用、RPM类型及其吸收进行概述,并对蛋氨酸对反刍动物肝脏健康的影响与机制展开综述,旨在为RPM在反刍动物生产中的应用提供科学依据和理论参考。
姜佳利席敏杰王博张瑞阳
关键词:过瘤胃蛋氨酸反刍动物肝脏糖代谢脂代谢
甲硫氨酸代谢轴关键酶在多发性骨髓瘤中的作用
2024年
目的探讨甲硫氨酸代谢轴是否可作为多发性骨髓瘤(MM)潜在治疗靶点。方法选取血液内科就诊的10例多发性骨髓瘤患者(新诊断MM组与治疗反应MM组),另外选择同期在广东医科大学附属医院自愿捐献骨髓上清液的健康者5例(Ctrl组)。采用超高效液相色谱质谱联用技术(UPLC-MS/MS)对3组人群骨髓上清液进行靶向定量代谢组学分析;利用代谢组学数据进行多变量统计分析。分析NCBI GEO数据库中人类MM相关高通量基因芯片数据,利用在线工具Cell Ranger和Seurat软件、生物信息工程分析技术筛选芯片数据中共同的差异基因,筛选MM中甲硫氨酸代谢轴相关的发病关键基因。结果检测骨髓上清液发现MM患者中甲硫氨酸和半胱氨酸水平显著升高(P<0.05)。筛选出参与甲硫氨酸循环的酶有甲硫氨酸腺苷转移酶2A(MAT2A)、腺苷同型半胱氨酸酶(AHCY)和精氨酸甲基转移酶1(PRMT1),发现与MM进展相关。结论MAT2A、AHCY和PRMT1这些关键酶可能通过调节甲硫氨酸代谢,影响MM的生物学行为和疾病进程。
袁鑫赵国鑫岑玉蓉郝嘉懿李宁冯锦山黄金棋何红华
关键词:多发性骨髓瘤生物信息学
晶体蛋氨酸和蛋氨酸羟基类似物在水产饲料中的应用研究进展
2024年
水产饲料常需添加氨基酸产品以满足水产动物的营养需求。蛋氨酸(Met)是水产动物的第一限制性氨基酸,参与机体多种代谢过程。蛋氨酸添加剂通常包括晶体蛋氨酸和蛋氨酸羟基类似物两类,并以DL-蛋氨酸(DL-Met)、L-蛋氨酸(L-Met)和蛋氨酸羟基类似物钙盐(MHA-Ca)较为常见。晶体蛋氨酸和蛋氨酸羟基类似物分别具有不同的理化性质,在动物体内有不同的吸收转化过程,二者的应用效果也存在较大争议。文章综述了晶体蛋氨酸和蛋氨酸羟基类似物的理化性质、生物代谢、应用和生产等现状,以期为蛋氨酸添加剂在水产饲料中的后续发展和应用提供参考。
郑路郎咸东邵庆均
关键词:蛋氨酸蛋氨酸羟基类似物代谢过程
Determination of Carbon and Nitrogen Isotope Fractions in Asparagine, Aspartic Acid, Threonine and Methionine
2024年
The nomenclature for compounds that are modified with isotopes is growing every day. Compounds can be modified with isotopes either individually, in a functional group or groups, or completely with all atomic centers of the element. This diversity of isotope-modified compounds increases the range of researches that can be studied using them. Compounds modified with isotopes of carbon-13 or nitrogen-15 can be converted into carbon monoxide, carbon dioxide and molecular nitrogen. Currently, only the average value of carbon-13 or nitrogen-15 isotopes can be determined. However, by directly determining the atomic share of these isotopes in organic compounds modified with isotopes, information about the isotopic centers of the element can be obtained. The atomic fraction of an element is defined as a single carbon or nitrogen isotope-modified center or centers, or all centers that are isotope-modified with that element at the same time. Carbon-13 or nitrogen-15 isotopes’ atomic fraction can be determined molecularly or with fragment ions of different elemental content, or both. This makes the method self-verifying, increasing the accuracy and reliability of the results obtained. Amino acids, such as asparagine, aspartic acid, methionine, and threonine, are essential for the human body. This proposed method of isotopic analysis will increase the possibilities for scientific research using these compounds.
Lamzira PharulavaLevani EliashviliVakhtang BetlemidzeBachana Sulava
关键词:ASPARAGINETHREONINEMETHIONINE
蛋氨酸构型和添加水平对黄羽肉鸡生长性能、胴体品质、肉品质和血浆蛋氨酸代谢相关酶活性的影响
2024年
本试验旨在研究饲粮添加不同构型和不同水平蛋氨酸对中速型黄羽肉鸡生长性能、胴体品质、肉品质和血浆蛋氨酸代谢相关酶活性的影响,以确定中速型黄羽肉鸡蛋氨酸需要量以及L-蛋氨酸相对于DL-蛋氨酸的相对生物学效价。试验分为2个独立的饲养试验,分别选用31和61日龄健康且发育良好的科朗麻黄母鸡各1584只,随机分为11个组,每组6个重复,每个重复24只鸡。各组分别饲喂在基础饲粮中添加0(对照组,2种构型共用)、0.04%、0.08%、0.12%、0.16%和0.20%L-蛋氨酸或DL-蛋氨酸的饲粮。试验期共计50 d。结果表明:1)与对照组相比,饲粮添加蛋氨酸显著提高黄羽肉鸡31~60日龄平均日增重和平均日采食量(P<0.05),并显著降低料重比(P<0.05),且L-蛋氨酸的效果优于DL-蛋氨酸(P<0.05);饲粮添加蛋氨酸对61~80日龄生长性能无显著影响(P>0.05)。2)与对照组相比,饲粮添加L-蛋氨酸显著提高60日龄黄羽肉鸡胸肌率(P<0.05),饲粮添加DL-蛋氨酸有提高60日龄胸肌率的趋势(P=0.06);饲粮添加蛋氨酸对80日龄胴体品质无显著影响(P>0.05)。3)与对照组相比,饲粮添加DL-蛋氨酸和L-蛋氨酸均显著降低80日龄黄羽肉鸡胸肌剪切力(P≤0.05)。4)与对照组相比,饲粮添加DL-蛋氨酸和L-蛋氨酸均显著降低80日龄黄羽肉鸡血浆甜菜碱-同型半胱氨酸甲基转移酶(BHMT)活性(P<0.05)。与DL-蛋氨酸相比,饲粮添加L-蛋氨酸显著提高60日龄黄羽肉鸡血浆BHMT活性(P<0.05),显著降低血浆甲硫氨酸腺苷转移酶活性(P<0.05);饲粮添加L-蛋氨酸显著提高80日龄血浆胱硫醚-β-合成酶活性(P<0.05)。综上所述,在本试验条件下,以平均日增重为评价指标,根据二次曲线回归分析得出,中速型黄羽肉鸡31~60日龄饲粮DL-蛋氨酸和L-蛋氨酸最适添加水平分别为0.16%和0.20%,相应饲粮蛋氨酸需要量分别为0.42%和0.46%;61~80日龄饲粮蛋氨酸需要量不高于0.23%。以平均日增重为评价�
林厦菁林泽玲苟钟勇范秋丽董晓丽邓远帆叶金玲王一冰张赛阮栋蒋守群
关键词:黄羽肉鸡DL-蛋氨酸相对生物学效价
Methionine sources and genotype affect embryonic intestinal development,antioxidants,tight junctions,and growth-related gene expression in chickens
2024年
Methionine(Met)is an essential and first limiting amino acid in the poultry diet that plays a significant role in chicken embryonic development and growth.The present study examined the effect of in ovo injection of DL-Met and L-Met sources and genotypes on chicken embryonic-intestinal development and health.Fertilized eggs of the two genotypes,TETRA-SL layer hybrid(TSL)—commercial layer hybrid and Hungarian Partridge colored hen breed(HPC)—a native genotype,were randomly distributed into four treatments for each genotype.The treatment groups include the following:1)control non-injected eggs(NoIn);2)saline-injected(SaIn);3)DL-Met injected(DLM);and 4)L-Met injected(LM).The in ovo injection was carried out on 17.5 d of embryonic development;after hatching,eight chicks per group were sacrificed,and the jejunum was extracted for analysis.The results showed that both DLM and LM groups had enhanced intestinal development as evidenced by increased villus width,villus height,and villus area(P<0.05)compared to the control.The DLM group had significantly reduced crypt depth,glutathione(GSH)content,glutathione S-transferase 3 alpha(GST3),occludin(OCLN)gene expression and increased villus height to crypt depth ratio in the TSL genotype than the LM group(P<0.05).The HPC genotype has overexpressed insulin-like growth factor 1(IGF1)gene,tricellulin(MD2),occludin(OCLN),superoxide dismutase 1(SOD1),and GST3 genes than the TSL genotype(P<0.05).In conclusion,these findings showed that in ovo injection of Met enhanced intestinal development,and function,with genotypes responding differently under normal conditions.Genotypes also influenced the expression of intestinal antioxidants,tight junction,and growth-related genes.
James K.LugataSawadi F.NdunguruGebrehaweria K.RedaXénia E.OzsváthEszter AngyalLevente CzeglédiGabriella GulyásRenáta KnopJános OláhZoltán MészárRita VargaBrigitta CsernusCsaba Szabó
关键词:DL-METHIONINEL-METHIONINEPOULTRY
Feeding Practices and Use of Lysine and Methionine in Pigrationing on Intensified Pig Breeding in the West Center and Hauts Bassins Regions of Burkina Faso
2024年
The aim of the study was to assess feeding practices and the use of lysine and methionine in pig rationing on intensified and semi-intensive pig breeding in the Koudougou and Bobo-Dioulasso areas. To this end, a cross-sectional survey was carried out on 87 breeding in these towns. A Discriminant Factorial Analysis (DFA) confirming a k-means classification of the data collected was used to retain 71 breeding divided into three breeding classes: Class A (32.4% of breeding), Class B (14.08%) and Class C (53.52%). The results show that the majority of pig breeders were men between the ages of 36 and 59. Average herd sizes were 35 ± 28;79 ± 42 and 89 ± 21 pigs for Classes A, B and C respectively. The main breeds of pig found on the breeding were crossbred, Large white, local, Landrace and Duroc. Class A (26.1%), B (30%) and C (15.8%) breeders were familiar with both lysine and methionine. Class A breeders distributed feed staggered (65.2%) and in rations (34.8%). Lysine (13%) and methionine (8.7%) were purchased at 5250 FCFA/kg. Those in class B distributed feed staggered (50%) and in the form of rations (50%), in which they incorporated lysine (30%) and methionine (30%) purchased at a cost of 2500 FCFA/kg and 3000 FCFA/kg respectively. Rationing and staggered feeding were practiced by 23.7% and 76.3% of Class C breeders respectively. Only lysine purchased at 3400 FCFA/kg was incorporated into rations by 10.5% of breeders. The high cost of lysine and methionine was incriminated by Class A (100%), B (33.3%) and C (50%) breeders. In conclusion, intensive pig breeding, the practice of rationing and the incorporation of the amino acids lysine and methionine are of ascending importance from classes C, A to B. The high cost of feedstuffs, particularly lysine and methionine, compromises their use in rations, which could have a negative impact on expected breeding performance. The screening and use of feeds rich in and/or enriched with these amino acids, through the development or adaptation of technologies, could improve
Isidor Zangbéwindin OuedraogoTimbilfou KiendrebeogoAboubacar ToguyeniChantal Yvette Kabore-Zoungrana
关键词:LYSINEMETHIONINE
Improvement of glucose metabolism in middle-aged mice on a high-fat diet by whole-grain highland barley is related to low methionine levels
2024年
Methionine restriction(MR)is an effective dietary strategy to regulate energy metabolism and alleviate oxidative stress and inflammation in the body,especially in the middle-aged and elderly population.However,the high methionine content of meat products makes this dietary strategy impossible to combine with protein supplementation and MR.Highland barley(HB),a low-methionine cereal,not only provides the body with protein but also has improved glucose metabolism and antioxidant and anti-inflammatory properties.Therefore,this study evaluated the feasibility of HB as a source of methionine-restricted dietary protein and the potential mechanisms.Middle-aged C57BL/6J mice were fed a control diet(CON),a high-fat diet(HFD),a whole-grain HB high-fat diet(HBHF),or a HBHF+methionine diet(HBHFmet)for 25 weeks.The results showed that the HBHF could keep the body weight,fasting glucose,insulin,homeostasis model assessment of insulin resistance(HOMA-IR),blood lipids,inflammation,and oxidative stress of HFD mice at normal levels.Compared with the HFD groups,HBHF inhibited pancreatic cell apoptosis and improved insulin secretion while improving hepatic and skeletal muscle glucose metabolism.However,these efficacies were attenuated in HBHFmet group mice.These findings suggest that HBHF has an MR strategy.
Chuanxing FengYueting GeBowen LiXiangrong ChengXue TangJianjin ZhuYuge JiangYonghui ShiGuowei Le
Effects of methionine supplementation in a reduced protein diet on growth performance, oxidative status, intestinal health, oocyst shedding, and methionine and folate metabolism in broilers under Eimeria challenge
2024年
Background This study investigated effects of different methionine(Met)supplementation levels in a reduced protein diet on growth performance,intestinal health,and different physiological parameters in broilers under Eimeria challenge.A total of 600 fourteen-day-old Cobb500 male broilers were challenged with E.maxima,E.tenella,and E.acervulina,and randomly allocated in a 2×5 factorial arrangement.Birds received normal protein diets(20%crude protein,NCP)or reduced protein diets(17%crude protein,LCP),containing 2.8,4.4,6.0,7.6,and 9.2 g/kg of Met.Results On 6 and 9 days post inoculation(DPI),increasing Met level linearly improved the growth performance(P<0.05).Total oocyst shedding linearly increased as Met level increased(P<0.05).Duodenal villus height(VH):crypt depth(CD)in the LCP groups were higher on 6 DPI(P<0.01)while lower on 9 DPI(P<0.05)compared to the NCP groups.Jejunal CD and duodenal VH:CD changed quadratically as Met level increased(P<0.05).On 6 DPI,liver glutathione(GSH)and glutathione disulfide(GSSG)linearly increased as Met level increased(P<0.05).On 9 DPI,GSSG quadratically increased,whereas GSH:GSSG quadratically decreased as Met levels increased(P<0.05).The expression of amino acid transporters linearly decreased as Met level increased(P<0.05).The expression of zonula occludens 2 and claudin-1 linearly increased on 6 DPI whereas decreased on 9 DPI as Met level increased(P<0.05).The expressions of cytokines were lower in the LCP groups than the NCP groups(P<0.05).Interaction effects were found for the expression of IL-10 and TNFαon 6 DPI(P<0.05),where it only changed quadratically in the NCP group as Met level increased.The expression of Met and folate metabolism genes were lower in the LCP groups than the NCP groups on 9 DPI(P<0.05).The expression of these genes linearly or quadratically decreased as Met level increased(P<0.05).Conclusion These results revealed the regulatory roles of Met in different physiological parameters including oxidative status,intestinal health,and nutrient metabolism i
Guanchen LiuVenkata Sesha Reddy ChoppaMilan Kumar SharmaHanseo KoJanghan ChoiWoo Kyun Kim
关键词:COCCIDIOSISEIMERIAMETHIONINE
细菌中L-甲硫氨酸生物合成和调控机制
2024年
L-甲硫氨酸是一种必需氨基酸,在食品、饲料、化妆品和药品中具有广泛的应用。目前,L-甲硫氨酸是唯一无法用微生物发酵法工业化生产的必需氨基酸,近年来,利用代谢工程提升L-甲硫氨酸产量受到国内外研究人员的普遍重视。本文主要分析了细菌中L-甲硫氨酸的生物合成途径及调控机制,着重分析了从高丝氨酸生物合成甲硫氨酸的三个关键步骤(即酰化、硫化和甲基化),并进一步对L-甲硫氨酸的生物合成提出展望,以期为L-甲硫氨酸的工业化生产提供指导。
崔莹宋凯何亚文
关键词:L-甲硫氨酸生物合成

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